Effect of Cytokinin Concentrations on Shooting ability of plantain Genotypes; ‘Efol’ and Pita 14.
This study was aimed at determining the effect of different6-Benzylaminopurine (BAP) on
shooting abilities of two plantain cultivars („Efol‟ and Pita 14). The shoot tips of each
genotype were isolated using appropriate protocol for shoot tip isolation under aseptic
conditions. After which they were cultured on media supplemented with BAP at four
different concentrations being 0.00 mg/l, 0.0025 mg/l, 0.005mg/l and 0.0075 mg/l,and on
three growth environments (growth room, bench top and screen house). The results
indicated that the number of replication increased at 0.0025 mg/l and 0.005mg/l, with
optimum replication observed at 0.005 mg/l for „Efol‟ genotype in growth room and screen
house and Pita 14 genotypein growth room. Significant difference was determined at P <
0.05 and observed for „Efol‟ cultured in growth room and screen house and also for Pita 14
cultured in growth room. The research has shown that BAP concentration at 0.005 mg/l is
optimum for both genotypes and bench top is not conducive for in vitro multiplication.
Keywords: Cytokinin, concentration, shooting, genotypes and Efol, Pita 14